To guarantee a smooth in-orbit space gravitational wave detection for the Taiji mission, a semi-physical simulation test of inter-satellite laser interference is carried out. The semi-physical simulation test consists of three aspects: the establishment of the inter-satellite laser link, interferometry of the inter-satellite ranging, and simulation of the space environment. With the designed specifications for the semi-physical simulation platform, the test results for the inter-satellite laser interference can be obtained. Based on the semi-physical simulation test, the risks of inter-satellite laser interference technology can be mitigated, laying a solid foundation for the successful detection of in-orbit gravitational waves.